Lab Protocols

Cryolysis of Yeast cells using a PM-100 grinder

Cryolysis of mammalian cells using a PM-100 grinder

Harvesting Yeast cells and making cryo noodles

Conjugation of Dynabeads

Single-step Affinity Purification

Western analysis of ssAP samples

In-solution trypsin digest

On-bead trypsin digestion

MS sample preparation and run 

Video Protocols (see also NCDIR)

 Harvesting Yeast cells and making cryo noodles

Cryogrinding (PM-100)

For questions regarding these or any other protocols or techniques used in the   Oeffinger Lab,  please contact us: oeffingerlab[at]gmail.com, marlene.oeffinger[at]ircm.qc.ca or lisbeth-carolina.aguilar[at]ircm.qc.ca

Plasmids and Strains

For published plasmids and strains, please contact us: oeffingerlab[at]gmail.com or lisbeth-carolina.aguilar[at]ircm.qc.ca