Studying Protein Neighbourhoods

While the components of many RNPs, pre-ribosome, and mRNP, have been identified, the near-neighborhood of these proteins has not. It is still unclear which proteins interact directly within these macromolecular assemblies. We have developed a targeted crosslinking-MS approach that allows us to determine closely arranged proteins within large RNP complexes. Moreover, we are able to distinguish dynamic exchanges of interactors. This approach is completed with classic crosslinking-MS techniques and computational modeling in order to gain a more comprehensive picture of intra-complex protein interactions, which will allow us better understand functional relationships between RNP components.


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