The yeast S. cerevisiae has ~6000 genes, ~5% of which contain introns, whose transcripts are processed, folded and quality controlled by only ~250 proteins. A question we are interested in the lab is whether all of these transcripts are processed by the same set of nuclear proteins. We have recently shown that different mRNAs in yeast are preferentially exported from the nucleus to the cytoplasm via different subsets of nuclear pores – with long, intron-poor mRNAs with longer PolyA-taisl preferentially found with nuclear pores that contain a ‘basket’ structure on their nuclear face, which, in yeast, is formed by the myosin-like proteins Mlp1/2 (human TPR)Using proteomics, RNomics, and biochemical approaches, we are studying the nuclear processes and proteins that could facilitate such selectivity of nuclear pore association and nucleo-cytoplasmic transport, including the differential mRNA maturation factors.

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